Jade Sharp, 23, started her fight after getting fed up with drivers from the private taxi firm refusing to take her golden retriever Brodie.
She successfully got Mohamed Mohamoud, 51, convicted and landed with a £1,550 bill at Hammersmith Magistrates' Court.
He is one of eight Uber drivers Transport for London (TfL) have brought cases against on Miss Sharp's evidence, four of which have already been found guilty.
Mohamoud, of Tulse Hill, south east London, cancelled on Miss Sharp, who was with her blind friend Vanessa Smith, 21, after she explained that the five-year-old working dog would be coming with them.
The pair were leaving a concert at Fairfield Halls, in Croydon, south London, at around 9.30pm on May 7 when she booked a minicab using the Uber app.
Miss Sharp, who was born blind, gave him a call to say Brodie would be joining them because Uber has no option in its app to alert drivers.
She told Mohamoud: 'I have got a guide dog travelling with me.'
He said: 'I don't take pets.'
And she replied: 'He is not a pet, he is a working dog. He is my eyes.'
She then threatened to report him to Uber, to which he replied: 'Fine, report me to Uber.'
Miss Sharp told the court: 'About ten seconds later I got a notification on my phone that the trip had been cancelled by the driver.'
He claimed he did not understand Brodie was a guide dog and refused pets on the grounds it would set off his allergies.
But District Judge Jeremy Coleman said he had not told the truth and ordered him to pay a total of £1,546 in costs and fines under the Equality Act 2010.
Judge Coleman said: 'The evidence they gave to me is consistent. I find it to be reliable and it certainly had a ring of truth about it.
From the Daily Mail: